FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60126022
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA726780.12087611583550296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT35950.013.9577811
GGGAGGT300350.013.22127153
TCGTGTA40300.012.50529914
GTTGTCG27150.012.17977726
GGAGGTC323500.011.9067294
GTCGTGT42650.011.76400913
ATAGGGG250000.011.45101938
AGCTTAG255650.011.20799945
GGCATAG257200.010.98040935
CGTCGTG46450.010.80245912
GTCGCCG52400.010.56694144
GTCACTC353800.010.5575578
GGTCACT361100.010.4874417
GAGCTTA280000.010.17703244
TGGGAGG299250.010.1711732
GTAGGGA52900.010.03559518
AGCGTCG51950.09.91882610
CACCGAA394950.09.716945
ATATAGC48200.09.6168556
TGGCATA297500.09.5831934
Produced by FastQC (version 0.11.5)