FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46226906
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26900.012.88041826
CGTAACG15950.012.69724842
ATAGGGG133150.011.390820538
TAATCGG90550.010.9807714
GGCATAG131100.010.9668735
GTAACGA90200.010.92482718
GGGAGGT194750.010.8926433
GGTAACG92900.010.6800717
AATCGGT97400.010.6473845
GGAGGTC191800.010.6027584
ATAATCG97950.010.4727183
ATCGGTT100350.010.1773066
ATATAGC48700.010.1622096
GTGTCGA101900.09.97991227
TACGGTT31900.09.87465535
GAGCTTA152550.09.778542544
GAGGTCA215200.09.6485135
TGTCGAG107200.09.59198228
AGCTTAG163600.09.5581645
TGGCATA152500.09.5460234
Produced by FastQC (version 0.11.5)