FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50254522
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG778570.15492536174157623No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG711280.1415355219178087No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA566980.11282168796670675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT32700.017.3394411
TCGTGTA36300.015.681807514
AGCTTAG261850.014.61632745
GTTGTCG34850.013.81633426
GTCGTGT41400.013.80433913
GGGAGGT403800.013.4287493
GAGGTCA398050.013.2382785
GGAGGTC394250.013.1719154
AGGTCAC398250.013.0847386
GTCACTC387650.013.059448
GGCATAG295900.012.85060535
GGTCACT396200.012.8230497
GAGCTTA299000.012.74006144
ATAGGGG306600.012.52688738
ACTCTCC414250.012.38381911
TGGCATA312900.012.1955734
GGTCGCC52550.012.03146343
CACTCTC434650.011.64729510
TCACTCT445850.011.6019779
ACCCGGC93600.011.44235
Produced by FastQC (version 0.11.5)