FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52863582
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1265800.2394465059140336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT264750.024.0742876
GACCGCG266500.023.91625
AGACCGC267150.023.8832764
CAGACCG276950.023.2168053
CCGCGTT275150.023.1792437
CGCGTTC276350.023.1192978
TCAGACC319850.020.447582
GCGTTCT330750.019.4392059
CTCAGAC369750.018.829751
TTCGGCG84400.018.15462744
TCGGCGC88250.017.4898145
CCGTACG94750.016.7873844
CGTACGC95650.016.5353415
CGTTCTC403400.016.1892710
GAACGAT397600.016.0994745
CTACACG68500.016.0617945
CGTTACG22750.015.42649221
GTTCCTA93350.015.32703315
TACGCCA105150.015.0618767
CGGGGCG105650.014.59170633
Produced by FastQC (version 0.11.5)