FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52863582
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG789380.1493239712738346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30250.016.81131726
GGGAGGT367450.015.3000883
AGCTTAG258650.015.19961145
ATAGGGG262350.015.09059438
GGCATAG260450.015.03473835
GGAGGTC374350.014.7978274
TGGCATA282650.013.9254434
GAGCTTA289400.013.59258144
GGTCACT403400.013.5886617
GTCACTC433250.012.8611878
ACTCTCC430900.012.74527211
TGTCGTT40250.012.69039128
CATAGGG325200.012.22260637
TGGGAGG338700.012.1898332
CACTCTC476750.011.59002410
CTGGGAG393250.011.5684291
GCATAGG343400.011.56040336
GCTCTGT496800.011.1677822
TCACTCT513850.011.13793859
ATATAGC53100.011.0978156
Produced by FastQC (version 0.11.5)