FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51751051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1181000.2282079256709202No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG862550.16667294351181391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC284150.020.429594
GACCGCG283400.020.3725035
ACCGCGT284600.020.3147836
GAACGAT289700.020.01731345
CAGACCG294350.019.8595893
CCGCGTT295600.019.5433127
CGCGTTC298800.019.3638
GCCGGCT247850.018.5939431
CGTTACG27000.018.23161121
TCAGACC340000.017.5902182
TCACGCT278500.017.3604078
CCGGCTT292100.017.1470262
GCGTTCT342200.017.0779429
TTCACGC300050.016.2942777
AGAACGA358650.016.27579344
CTCAGAC395000.016.2017881
TTCGACG25400.016.106976
AACGCTA293550.015.97497823
ACGCTCA311650.015.88874210
ACGCTAC298000.015.76024624
Produced by FastQC (version 0.11.5)