FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51751051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27600.010.83833626
AGGGATC173600.010.3361862
TCACCCG92200.09.6820363
ACCCGGC84750.09.6574245
TCAGACG88700.09.1514672
CAGGGAT226600.08.9338951
ATAGGGG116100.08.86445638
CACCCGG100450.08.8424
CAGACGT103800.08.8197311
GGCATAG110500.08.53921235
GGGATCA222050.08.4859493
CCGGACA97250.08.18551412
ACGGACA99000.08.18016418
ATAGCTC90900.08.15079839
GGGAGGT209700.08.1171683
AGCTTAG124450.08.05669445
CGGACAC97800.08.0475113
CGGCCCG113650.07.8552068
GGTCACT183450.07.83217337
TGTCGTT38500.07.831887728
Produced by FastQC (version 0.11.5)