FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42793046
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG693580.16207773571434947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG214100.015.70068245
GGGAGGT334700.014.7624573
GTCACTC325600.014.6844128
ATAGGGG231100.014.65273138
GGTCACT325000.014.6492147
GGCATAG234300.014.26054735
GGAGGTC359350.013.6433694
GAGCTTA248100.013.58531144
ACTCTCC360100.013.3775211
TGGCATA255100.013.15071134
CACTCTC377400.012.82391310
TCACTCT391250.012.5424789
AGGTCAC402750.012.1955176
TGGGAGG292850.012.1777232
CATAGGG284450.011.98363937
CTGGGAG348650.011.4677921
GAGGTCA436600.011.4509725
GCTCTGT424600.011.4301722
TCAGACG87600.011.4297822
GCATAGG298050.011.406630536
Produced by FastQC (version 0.11.5)