FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53713445
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG721310.1342885380001227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG222850.016.29691345
GTTGTCG28950.015.93356226
ATAGGGG243700.014.91514438
GGCATAG244950.014.77381735
GGGAGGT366850.014.6712073
GGTCACT367750.014.1289487
GTCACTC371100.014.0929648
GAGCTTA265100.013.70962144
GGAGGTC402900.013.2804664
TGGCATA276350.013.1602234
AGGTCAC423300.012.7258726
ACTCTCC420250.012.39823611
CATAGGG298850.012.17784437
GAGGTCA450300.012.1523975
TCACTCT447850.011.8638549
TGGGAGG323600.011.8144092
TGTCGTT40300.011.613996528
GCATAGG316550.011.46765236
CACCCGG130650.011.257941
CACTCTC467050.011.21756810
Produced by FastQC (version 0.11.5)