FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46073345
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG658060.14282878744749267No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG508350.11033494529212064No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA500920.10872229919490325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG190600.016.5018345
GGCATAG206450.015.09669635
ACCCGGC96300.014.7940125
GAGCTTA218500.014.50273244
TGGCATA223700.013.95142934
ATAGGGG229550.013.79331238
ATAGCTC98400.013.63180939
TAGCGTA13200.013.46818837
TCACCCG110500.013.4026333
GGGAGGT363200.013.1871913
TCAGACG104200.012.981682
CATAGGG249150.012.64512537
CCGGACA119050.012.43028412
GGAGGTC361400.012.4170934
ACTCTCC365500.012.12619911
GGTCACT364250.012.1227947
CGGACAC121350.012.10195513
GCATAGG262900.011.98380936
GTCACTC373250.011.9320818
TAGCTCT113450.011.88296140
Produced by FastQC (version 0.11.5)