FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46409237
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT880070.1896325078561408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT178800.025.93440845
AGACCGC200550.023.2948764
CAGACCG205050.022.8933983
AGAACGA214400.021.64900844
AAGAACG232450.019.98729543
AACGCTA103550.019.728823
TACGGAG235850.019.59420234
ATACGGA240400.019.41988833
ACGCTAC106150.019.35154724
GACCGCG242350.019.2862285
AATACGG245000.019.04608532
CGCTACC111800.018.37358725
CAATACG257300.018.22304731
GCGTTCT260850.017.9922929
ACGGAGA259950.017.84686335
ACCGCGT263650.017.7926796
CCGCGTT266900.017.609747
CTCAGAC291450.017.6064551
CGCGTTC267200.017.5815498
CCCAATA271750.017.26233729
Produced by FastQC (version 0.11.5)