FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46409237
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG502740.10832757280624976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156400.015.50788945
ATAGGGG164800.015.07098838
GGCATAG166650.014.60443435
GAGCTTA179500.013.63755444
GGGAGGT268900.013.5851193
GTCACTC274050.012.7796868
ACTCTCC279500.012.64504711
CATAGGG198000.012.55438737
GGTCACT278800.012.5295467
TGGCATA199250.012.24881734
AGGTCAC297350.011.9979866
GCATAGG209450.011.878098536
TCACTCT309300.011.5648379
CACTCTC305100.011.46604910
GAGGTCA320650.011.2587495
GGAGGTC319900.011.2432464
AGCATAG104350.09.91827345
TAGGGGA352350.09.85847839
TGGGAGG272300.09.8259172
GCTCTGT354750.09.80266722
Produced by FastQC (version 0.11.5)