FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52040338
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA576900.11085631303931961No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG527420.10134830407903961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG58500.028.118496
TCGACGA64100.025.9428047
CGGTATA91400.024.49725320
GTTCGAC68350.023.937195
CGACGAG68950.023.6582648
AGTTCGA81800.020.6065924
GACCGCG88750.020.5396925
AGACCGC91700.020.0261974
ACCGCGT93350.019.6218786
ATGACGG110550.019.46146616
CAGACCG97000.019.2103943
GACGGTA120000.018.65874318
AACGACG29450.017.95804216
ACGGTAT128200.017.7461319
CCGCGTT103400.017.7147247
GTATAGA136400.017.54612522
CGAGGAT109700.017.25060811
ATAGAGT137750.017.16197224
GACGAGG117550.016.9394689
CCGGCTT215600.016.8786982
Produced by FastQC (version 0.11.5)