FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47605257
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT258800.012.9437813
AGCTTAG183350.012.44502145
GGAGGTC272800.012.2712854
GTTGTCG25650.012.01514326
ATAGGGG191150.011.95897938
GGCATAG189250.011.91162335
TGGCATA209100.010.83387834
GAGCTTA209500.010.82717244
ACTCTCC333600.09.82743411
CGTGGCG130450.09.802851
ATAACGC59100.09.70789539
CACTCTC338450.09.68658610
CATAGGG237800.09.65061137
GTCACTC338950.09.6192598
GGTCACT334300.09.6114297
TAACGCC62350.09.346724540
GCCACGA176650.09.18167632
GCATAGG252450.09.13520136
TCAGACG85150.09.0685892
ACATGCG176800.09.0233910
Produced by FastQC (version 0.11.5)