FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48439334
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT671750.13867862014783275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156900.021.2956264
CAGACCG166950.020.2291683
GACCGCG173600.019.1693125
ACCGCGT176550.018.912696
CCGCGTT187350.017.7975047
CGCGTTC190250.017.5163868
GCCGGCT234650.017.191471
TTCACGC242000.016.8350527
TTCGACG18350.016.6646966
CCGGCTT262300.016.6645092
TCACGCT244200.016.6024068
TCAGACC207500.016.4384942
ATAAACG99450.016.28338421
TAAACGC103050.015.45262222
CGTTACG17350.015.296761521
CGGCTTC295850.015.2611293
CTCAGAC253300.015.2599041
GCGTTCT221850.015.101569
ACGCTCA276050.014.90590510
CCGTACG63600.014.3183324
Produced by FastQC (version 0.11.5)