FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45592891
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG469070.10288226732540387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146400.015.122972545
ATAGGGG157350.014.05620338
GGCATAG161800.013.61400435
GAGCTTA176850.012.59543844
TGGCATA181750.012.28058334
GTTGTCG20050.012.23189826
GGTCACT250800.012.1471077
GGGAGGT269750.012.06953
GTCACTC258150.012.04538
CATAGGG186500.011.89542137
GCATAGG191450.011.51737136
AGGTCAC276250.011.4352726
ACTCTCC282450.011.04094411
GGAGGTC285350.011.0154114
GAGGTCA294650.010.9884525
TCACTCT315850.010.051439
TAGGGGA309950.09.84349339
CACTCTC314550.09.79974910
TGCCCGT126600.09.33058244
AGGGGAG338550.09.28442240
Produced by FastQC (version 0.11.5)