FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41653815
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA730050.17526605906325748No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG694890.16682505552012464No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG573150.13759844086309983No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG476230.11433046408834341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31350.016.65082626
GGGAGGT259050.014.2181673
GGAGGTC257850.013.9527524
ATATAGC46300.013.7039786
AGCTTAG202250.013.09382345
GTCGTTC40200.012.81758429
GGCATAG211600.012.66436735
ATAGGGG215300.012.63483838
TGTCGTT43300.012.15975328
GTCACTC307350.011.4786648
ACTCTCC310600.011.402019511
GCTAAGT46700.011.322165514
GAGCTTA236550.011.28080944
TGGCATA239250.011.18205234
TGGGAGG256050.011.1246782
CGTTCCC49450.010.82947531
TCGTTCC47400.010.8231430
AGGGATC192200.010.6762812
CACTCTC330700.010.61375210
CAATATA67200.010.3458874
Produced by FastQC (version 0.11.5)