FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45305501
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG137950.014.10295245
ATAGGGG147300.013.36723838
GGCATAG146850.013.23936135
GGGAGGT219200.013.12836553
GAGCTTA161550.012.02850644
GGAGGTC240750.011.6542584
TGGCATA172100.011.47933934
CATAGGG176200.011.18752837
GGTCACT246950.011.0353337
GTTGTCG20000.010.91274326
GCATAGG185500.010.6386136
ACTCTCC264550.010.43989111
GTCACTC263400.010.4145428
TAATCGG32050.09.6178284
AGGGATC122500.09.4796182
TGGGAGG227350.09.4038532
ACCCGGC63250.09.3556095
TCACTCT302200.09.3379799
TCAGACG70300.09.1556232
TAGGGGA293100.09.03646339
Produced by FastQC (version 0.11.5)