FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42667170
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG793700.18602124303064863No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT545160.12777036770894343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT298500.018.819788
GCCGGCT284150.018.489781
CCGGCTT326900.018.4373342
TTCACGC305700.018.339737
AACGCTA314000.017.48209623
ACGCTAC315300.017.42830524
TCGGCGC103950.017.42279445
CGGCTTC362750.017.1237683
CCGTACG107600.017.1033574
ACGCTCA257450.017.08418310
CGCTACC323450.016.98619725
CGTACGC109800.016.8426255
AAAACGC328300.016.76834921
GAAAACG332300.016.54593320
CGTTACG14550.016.39636621
TCCGTAC116150.016.1348933
AAACGCT343100.016.09771322
TACGCCA117000.015.8638447
GTCCGTT30350.015.6495681
CCCGTAT29150.015.5215851
Produced by FastQC (version 0.11.5)