FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42667170
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG576870.13520231128523405No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA497330.11656034370219538No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG494760.11595800705788549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT285750.013.9826793
AGCTTAG201750.013.89049145
TCAGACG107400.013.7412632
GGAGGTC292400.013.3197214
GGCATAG211450.013.23838335
ATAGGGG221150.012.8636438
GTCACTC300200.012.8395158
GGTCACT304250.012.6464157
GAGCTTA226100.012.41442644
TGGCATA227950.012.30914134
CGTGGCG206400.011.5427771
TGGGAGG256900.011.5331722
TAGCGTA6900.011.41517237
TCACTCT354900.011.145899
GTGGCGA215650.011.0477432
CATAGGG262400.010.82328337
ACTCTCC366750.010.669188511
TGGCGAC223100.010.6184843
AGGTCAC380000.010.5813936
GCATAGG271150.010.45685336
Produced by FastQC (version 0.11.5)