FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51503482
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG608720.11819006722691097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG188200.015.37309545
GGCATAG209900.013.83022135
ATAGGGG217500.013.48163238
GAGCTTA219000.013.25458544
GGGAGGT345800.013.2126613
GGAGGTC341050.012.86253454
TGGCATA228600.012.73828434
CATAGGG241150.012.08475337
TAGCGTA6750.012.01128637
ACTCTCC371850.011.6106311
GCATAGG255150.011.44809136
ACCCGGC86350.011.4055
GGTCACT390250.010.958497
AGCATAG123750.010.54263745
TGGGAGG299000.010.4603812
CACTCTC413700.010.45783710
TAGGGGA403650.010.37764439
ATAGCTC88250.010.23341239
GTCACTC429100.010.1716328
GCTCTGT425100.010.09723222
Produced by FastQC (version 0.11.5)