FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48616259
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG805820.16575113276404094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA199400.021.0993923
TTCGACG24950.020.919396
ACGCTAC203400.020.7065824
CGCTACC209200.020.08945825
AAAACGC218550.019.38438821
GCCGGCT222100.019.2864911
TCACGCT229000.019.1767318
GAAAACG222500.018.98962420
AAACGCT229850.018.42163322
CGTTACG16600.018.02586621
CCGGCTT258250.018.0081752
GTCCGTT39300.017.8127441
CGGTATA41950.017.8056120
TTCACGC248000.017.671267
ACGCTCA255000.017.32730910
CGGCTTC291300.016.5520463
CACGCTC271250.016.1648739
TCGACGA36450.014.9364727
CGTGGTT287000.014.70902641
GTTTTCG285700.014.6869345
Produced by FastQC (version 0.11.5)