FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48616259
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG146200.012.93061745
TCAGACG126000.011.7489682
GGCATAG161950.011.53243635
ATAGGGG168000.011.34500638
GAGCTTA168650.011.19586544
GGTCACT230750.010.7441327
TGGCATA179100.010.62877234
GTCACTC235450.010.5583478
CATAGGG186100.010.19317737
ACTCTCC250550.010.11083611
GGGAGGT271350.010.0985553
GGAGGTC265600.09.7835324
GCATAGG195650.09.70711336
GAGGTCA274150.09.6507345
GTTGTCG20500.09.54822726
CGTGGCG236100.09.5293771
CAGACGT158150.09.346263
ATAGCTC65500.09.34413339
GTGGCGA243000.09.1658622
GCACCGA215050.08.88485344
Produced by FastQC (version 0.11.5)