FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44993842
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG781520.1736948802905073No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA596200.13250702173866372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT337750.015.3625083
GGAGGTC354950.014.6443544
AGCTTAG259900.014.63663645
CACCGAA269200.013.73799545
GCACCGA275950.013.36932344
GGCATAG281450.013.35392335
GTTCTAT283350.013.2523491
ATAGGGG289600.013.11637538
GAGCTTA297450.012.84188444
TGGCATA299900.012.65963534
GTCACTC408050.012.4087658
TGGGAGG320950.012.3961932
GTTGTCG20900.012.38043526
ACTCTCC417950.012.16925311
TCTATAG321000.011.7920423
GGTCACT429100.011.752657
CTGGGAG374950.011.3109141
ATAGTGT342500.011.1644026
CACTCTC458450.011.13838110
CTATAGT338700.011.1300294
Produced by FastQC (version 0.11.5)