FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48138652
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1341640.27870327569621184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC286450.023.0678224
CAGACCG299750.022.1793983
GAACGAT298700.021.81274245
GACCGCG308200.021.3881365
ACCGCGT318500.020.7529726
CCGCGTT325450.020.3025827
CGCGTTC328150.020.1150518
TCAGACC347350.019.4085372
GCGTTCT351500.018.8556219
CTCAGAC386500.018.665631
AGAACGA354500.018.45547144
AATACGG378600.017.26480532
TACGGAG380150.017.14126434
ATACGGA381850.017.09478833
AAGAACG386050.016.92490443
CCGTACG61650.016.860114
CAATACG391950.016.71581331
ACGGAGA403850.016.16318335
CCTCTCA420100.016.06340219
CGTACGC64900.015.6686535
Produced by FastQC (version 0.11.5)