FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42405351
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA550160.12973834363498135No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG536000.12639914241011704No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG509100.12005560336005709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT244000.015.4181013
GGAGGTC269250.014.0638354
GTTGTCG16500.013.90180726
AGCTTAG208200.013.1624645
ATAGGGG210200.013.06036738
GGCATAG207850.013.03879935
TGGCATA224700.012.23159134
GAGCTTA227950.012.04247644
GGTCACT299950.011.9596797
TGGGAGG248450.011.7185692
TATCCTA108150.011.67489055
ACTCTCC315450.011.50136711
GTCACTC317750.011.3819878
ATCCTAG115550.011.2379866
TCAGACG83250.011.0354742
TGTATCC115250.010.9979493
CATAGGG255250.010.8290237
CTGGGAG303650.010.8221631
CCTAGGC114400.010.4264778
GTATCCT118950.010.3716914
Produced by FastQC (version 0.11.5)