FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51125628
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT936460.18316841017581242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC234200.021.6243464
GACCGCG232150.021.5535
CAGACCG240750.021.1855453
ACCGCGT242750.020.704546
CCGCGTT251950.019.9304377
CGCGTTC258250.019.4529468
GAACGAT256450.019.19531245
TCAGACC297700.017.7854922
GCGTTCT296200.017.0213419
CTCAGAC345600.016.8444731
AGAACGA305500.016.21648244
TACGGAG300300.016.10169634
AATACGG306550.016.04500232
ATACGGA309450.015.80770833
GTCCGTT38950.015.2521521
CCGTACG30150.015.2228954
CAATACG325750.015.22259831
AAGAACG329450.015.00428743
ACGGAGA327150.014.82145635
CGTTACG21400.014.72109121
Produced by FastQC (version 0.11.5)