FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51125628
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG25100.010.93524626
ATAGGGG133050.010.70448238
GGGAGGT193750.010.3520443
GGCATAG142650.09.699835
GGAGGTC214400.09.1615214
TAATCGG47200.09.152454
AGCTTAG156400.08.94817145
AGGGATC143350.08.7157892
GGTCACT214950.08.687247
GTAACGA44750.08.59786318
GTCACTC221150.08.4946398
GAGCTTA169000.08.4541344
AATCGGT54100.08.35942655
TGGCATA171800.08.22408234
ATCGGTT52900.08.208366
CGTAACG11050.08.144793542
TGTCGTT35300.07.966865528
CATAGGG176450.07.867380637
ATAATCG55450.07.83512453
CAGGGAT195300.07.80448341
Produced by FastQC (version 0.11.5)