FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49265385
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1387950.28172925066961313No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG660650.13410024097040954No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG528190.10721320862508231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC360100.020.0067794
CAGACCG363600.019.796893
GAACGAT361950.019.58744445
GACCGCG373600.019.114635
ACCGCGT376650.019.0036856
CCGCGTT391600.018.2781897
CGCGTTC393700.018.1692648
CGTTACG18800.017.59648721
TCAGACC424400.017.4752122
GCGTTCT412750.017.3851819
CTCAGAC455900.017.278371
AGAACGA430950.016.52572644
CGTTCTC478300.015.43525610
CCGGCTT283800.015.0293152
TTCACGC270500.014.8687987
CCGTACG37950.014.6487234
AATACGG487550.014.53708232
TCACGCT277000.014.4630488
TACGGAG485200.014.4423734
ATACGGA493250.014.407454533
Produced by FastQC (version 0.11.5)