FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49265385
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA697390.14155780980905763No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG552770.11220251298147776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT269600.013.4949773
GTTGTCG26150.013.42290326
AGCTTAG196350.012.55710845
GGAGGTC292550.012.1364494
GGCATAG206850.011.9554235
ATAGGGG208250.011.9509938
GAGCTTA224100.010.9414844
TGGCATA231850.010.64635734
ATATAGC40000.010.575866
TGGGAGG257550.010.4588732
GGTCACT330450.010.3306197
CACCCGG88250.010.2271761
GTCACTC335900.010.2166938
GAGGTCA365200.010.1039795
TCACTCT344250.09.9296289
TGTCGTT36800.09.78272828
AGGGATC174300.09.4765652
CTGGGAG316650.09.3683261
ACTCTCC368700.09.2905311
CATAGGG265100.09.2862737
Produced by FastQC (version 0.11.5)