FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49725916
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG638630.12843001222943787No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT544420.1094841571143707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG37200.029.63698832
CGTCCGC46500.023.85530533
TCGTATG46750.023.77074845
AGACCGC132150.020.2663544
ACCGCGT130850.020.2592476
GACCGCG131900.020.1340775
AGTCACG60050.018.99266628
GTCCGCA62200.018.5937434
CCGCGTT145550.018.2595217
CGCGTTC146150.018.119698
GCCGGCT233850.017.1789531
CGTCTGA67500.017.069316
ACACGTC68650.017.04400613
CAGACCG160050.016.930383
CCGCACA67500.016.43386836
CGTTACG20350.016.361821
CCGGCTT282750.016.1819422
TCACGCT270250.016.1430368
CCCGTAT33400.015.83496951
TTGCGTC69150.015.38766143
Produced by FastQC (version 0.11.5)