FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49725916
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG562720.11316433064802668No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA545040.10960884058928144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC50950.019.95915243
GCGTCGT54450.018.92422511
TCGTGTA55250.018.85381314
GTAGGGA62200.017.50722318
GTCGTGT59800.017.34414313
TCTCGGT67300.016.4140636
TGTAGGG67050.016.17361317
AGTGTAG69450.015.77658228
GTCGCCG71400.015.40840444
ATCTCGG79200.014.77160835
CGTCGTG71800.014.53934712
TGGTCGC82550.014.39018942
GTAGATC78750.014.02756531
AGCGTCG77750.013.832334510
GTTGTCG29200.013.56094426
AGCTTAG178550.012.75172545
CGGTGGT87250.012.63514639
ATAGGGG187300.012.45634438
TAGATCT86750.012.4226932
GGAGGTC248600.012.295354
Produced by FastQC (version 0.11.5)