FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42312210
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG710710.1679680640647227No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA638770.15096587958889407No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG573520.13554479900718966No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT555500.13128598104424233No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG535710.12660884411379128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG182500.015.23650845
ATAGGGG189400.014.59869638
GTTGTCG24900.014.547873526
GGGAGGT285750.014.4750813
GGCATAG190400.014.46304435
GTCACTC280550.014.0409338
GGTCACT278750.013.9459787
GAGCTTA203850.013.64077944
AGGTCAC301350.013.3254696
GGAGGTC308350.013.1866024
ACTCTCC298850.013.12087111
TGGCATA213250.013.0608934
GAGGTCA313400.012.9584255
CACTCTC319800.012.296514510
TCACTCT327050.012.1752589
CATAGGG233100.012.04521237
GCATAGG244000.011.488736
TGGGAGG265100.011.1774152
TGTCGTT34000.010.91874928
GCTCTGT369200.010.74378822
Produced by FastQC (version 0.11.5)