FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46964605
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG773940.16479218764854936No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA502260.10694436799798487No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT496090.10563061267096785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG70250.022.8358086
GTTCGAC60850.022.629295
TCGACGA78750.020.7138397
CGGTATA85800.020.42914220
CGACGAG71450.019.2403148
CCGGCTT252550.019.1813872
GCCGGCT233750.018.7832741
AGTTCGA76900.018.1403394
TTCACGC263450.017.6699547
TCACGCT267650.017.4935518
CGTTACG21450.017.20367221
GACCGCG152150.016.6512375
CGGCTTC295150.016.6339453
ACCGCGT153400.016.4712876
AGACCGC154100.016.4697324
GTCCGTT41550.016.4652441
CAGTTCG89350.015.9653353
CAGACCG162000.015.8889193
GTATAGA121850.015.56707822
ATGACGG112600.015.565927516
Produced by FastQC (version 0.11.5)