FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46964605
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA552160.11756939082102362No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG491570.10466818575393107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCTA123300.012.99620145
GGGAGGT234800.012.6072313
GGAGGTC240950.012.0891094
CGTAACG10250.011.41686942
ATAGGGG190300.011.21892238
GGTCACT263300.010.9829827
AGCTTAG194650.010.86858445
GGCATAG196450.010.80927435
TATATAC69900.010.753197
GAGGTCA285700.010.5806875
GTCACTC279450.010.4694638
ACTCTCC293150.010.18735811
TGGCATA212400.010.14580734
GAGCTTA212950.09.98742844
GTTGTCG28450.09.96204826
AGGTCAC309450.09.7391086
CATGCAT83750.09.6739279
TCACTCT310500.09.6617039
ATATATA87200.09.5244296
CACTCTC319000.09.305490510
Produced by FastQC (version 0.11.5)