FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47761178
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGT1166030.2441376131886864No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG1101850.23069992117866106No Hit
CCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGG968740.20283000557482062No Hit
CCTGGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGG967980.20267088052141427No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT902080.1888730633905219No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG848660.17768824713661793No Hit
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC761340.15940561600050987No Hit
CTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGG757170.1585325219574777No Hit
CTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTC686270.14368782947522776No Hit
CTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAA675700.14147473498245794No Hit
CTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTC630410.13199213804986132No Hit
CTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTTCTCGATGGGGGCTGGGA595720.12472891686214273No Hit
GTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCC593980.1243646042398703No Hit
GCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTG586150.12272519743964438No Hit
CCCTGCTGCCACCTGCTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAG585020.12248860361023757No Hit
CCCTGGCACTCATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTG582180.1218939784106665No Hit
CTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGC575820.12056235296373971No Hit
TTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGC558770.11699250801561051No Hit
CTCTTGTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCC550600.11528191369149228No Hit
GTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCT524170.10974813058421631No Hit
CTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTTCAGG516730.10819038006139631No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC512810.107369629785932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT152600.027.27786345
AGAACGA181700.022.9837444
AGACCGC195950.021.395734
TTCGGCG80450.021.36805344
GACCGCG196050.021.2929865
ACCGCGT199800.020.8933436
CCGCGTT204300.020.441697
CGCGTTC204350.020.4224918
CAGACCG206900.020.3830243
CGCGTGT13350.019.891771
AACGCTA65900.019.69896923
TCGGCGC86650.019.63117845
ACGCTAC67100.019.38020724
ATACGGA220700.018.97382433
TACGGAG220300.018.93677734
GCGTTCT224250.018.750659
AATACGG232850.018.0127732
ACGGAGA238450.017.61849235
CGCTACC73700.017.55308525
TCAGACC246650.017.4174042
Produced by FastQC (version 0.11.5)