FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47761178
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGG852000.1783875598713248No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT709480.1485474248562295No Hit
CCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAA697750.14609145528194467No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA539240.11290341289320796No Hit
CCTGAGGTCACATGCGTGGTGGTGGACGTGAGCCACGAAGACCCTGAGGTC535880.11219991265709568No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG516710.10818619255999087No Hit
GGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCG500650.10482362893143046No Hit
GTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAG489890.10257075317530905No Hit
CACAGGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGG489450.10247862814438957No Hit
CCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAG488900.10236347185574024No Hit
GCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGT484260.10139197152968045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTC96550.018.8335159
ACCCGGC84100.018.7482685
TCACCCG94750.016.9467563
GTCAGGA304000.016.5551011
CGGGACG91650.016.2747917
TCTCGGG96150.015.89185514
CAGCGCT118450.015.6552638
ACGTCTC95250.015.56363421
GCAGCGC118050.015.4596247
ACGGACA99050.015.39898918
TCAGTTC166900.015.2126643
CCGGACA106300.015.01061612
CGCTCTC121300.014.76854711
ATAGCTC103100.014.68550639
TCGGGAC103800.014.56719516
CACCCGG112450.014.3410394
ACACGGA108700.014.28313416
GGGACGT104700.014.22421518
CGGACAG106900.014.1840319
CTCAGTT218750.014.0421722
Produced by FastQC (version 0.11.5)