FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49676953
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT794070.15984676032767148No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG509150.1024921959283614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG21400.021.8830416
AGACCGC201000.021.350274
GACCGCG203300.020.9093785
ACCGCGT208050.020.496066
CAGACCG211150.020.4092663
CCGCGTT214650.019.8571347
CGCGTTC218750.019.49738
GAACGAT220500.019.46354945
CGTTACG28100.017.37901721
GCGTTCT254100.016.9442149
TCAGACC261500.016.9014952
GTTCCTA94150.016.78062815
AGAACGA267100.016.18606844
CTCAGAC307400.016.0993121
GCCGGCT176950.016.0453071
ATACGGA275250.015.62886333
TACGGAG274900.015.6078434
AATACGG274700.015.59452132
TCACGCT199000.015.3734048
TCGACGA30100.015.169387
Produced by FastQC (version 0.11.5)