FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49676953
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG552740.11126688869182455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27600.014.75442226
GGGAGGT282900.013.8334753
AGCTTAG215900.013.30959545
GGAGGTC289500.013.1839734
GGCATAG217450.013.08830535
ATAGGGG228300.012.64366638
GTCACTC297100.012.4517568
GGTCACT297050.012.3781617
GAGGTCA309800.012.29816155
AGGTCAC317200.012.0316816
GAGCTTA236400.012.03118244
TGGCATA241750.011.88438434
ACTCTCC316600.011.79070511
CACTCTC331750.011.22535710
TCACTCT340650.011.1702349
CATAGGG268600.010.63775537
GCATAGG276650.010.3038236
ATATAGC53050.010.05302056
TGGGAGG301400.010.0273172
CTGGGAG355950.09.4831941
Produced by FastQC (version 0.11.5)