FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49309203
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1081860.21940326230784948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT198550.026.65777245
AGACCGC229900.023.569564
CAGACCG234100.023.3683873
AGAACGA237800.022.38053344
AATACGG255700.021.02246732
GACCGCG259700.020.8103775
ATACGGA258200.020.76663233
TACGGAG258800.020.69242534
AAGAACG255850.020.67882343
CCGCGTT267350.020.2890227
CGCGTTC268450.020.2391628
ACCGCGT270400.020.0450346
CAATACG269950.019.96232831
TCAGACC287400.019.3795172
ACGGAGA287800.018.6382635
TTCGACG18950.018.4034466
CTCAGAC333000.018.0790041
CCCAATA301000.017.95310629
CGGAGAG302100.017.73390436
CCGTACG52000.017.569394
Produced by FastQC (version 0.11.5)