FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43522848
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT570820.13115410094486463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT151100.018.7018476
TTCGACG17450.018.6952766
GACCGCG151800.018.6007655
AGACCGC155150.018.2136364
CGCGTTC161300.017.4913168
CCGCGTT161750.017.4287457
CAGACCG165700.017.2033463
GTTCCTA106700.016.8055515
GAACGAT185250.015.42576745
CCTACTC116000.015.41941718
CTCCGAT44250.015.2156191
GCACTAC65300.015.0915325
CGTTACG14850.014.84772721
ACGCGTC17950.014.7916940
TCAGACC200100.014.6056512
CTCCGTT64100.014.438281
GCGTTCT198900.014.3318159
AGACGCT207200.014.25882341
GTTCGAC22250.014.2576495
CCCGTAT30700.014.1563281
Produced by FastQC (version 0.11.5)