FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43522848
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG503460.11567717259679329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33750.015.93331626
AGCTTAG180050.013.89615745
GGAGGTC244700.013.8659444
GGGAGGT246700.013.8356153
ATAGGGG190350.013.26239738
GGCATAG188650.013.22686135
GAGCTTA206200.012.29754244
TGGCATA208750.012.01795234
TGTCGTT48950.011.30744628
ACTCTCC299950.011.28186911
GTCACTC305200.011.0730548
CATAGGG226950.011.01452937
GGTCACT301400.011.0036387
TCGTTCC51850.010.97877430
TATAGCT57600.010.9765517
GCTAAGT52950.010.83568314
GTCGTTC53150.010.71024329
ATATAGC57800.010.6660796
GCATAGG235350.010.63096436
CACTCTC325250.010.51497810
Produced by FastQC (version 0.11.5)