FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48457771
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTTG732640.1511914363539338No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT702830.14503968826795605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT210450.023.1414621
AACGCTA231750.022.44104223
ACGCTAC234800.022.15914224
CGCTACC241150.021.6409425
AAAACGC252700.020.63385821
TCACGCT262900.020.329698
GAAAACG258250.020.22526220
CCGGCTT273200.020.0500162
AAACGCT263100.019.85250922
TTCACGC274550.019.5817457
ACGCTCA284950.018.97745910
GAACGAT179700.018.89615245
CGGCTTC316500.018.0815943
TTCGACG17700.017.9199566
AGACCGC195150.017.8210894
CGTGGTT293500.017.71508241
CGTTACG19450.017.69473521
TTCGGCG90800.017.30786344
CAGACCG205350.017.023533
CACGCTC323600.016.7525449
Produced by FastQC (version 0.11.5)