FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48457771
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG166100.011.90675545
GGGAGGT248100.011.5148093
TATCCTA99000.011.432725
GGCATAG169400.011.19296435
ATAGGGG171750.011.09595738
GGTCACT238500.010.9316677
TAGGCTA100150.010.831799510
GTCACTC244000.010.7672658
GTTGTCG20450.010.69133126
CCTAGGC105850.010.6493288
ATCCTAG120050.010.4562646
CGTGGCG143600.010.4426421
ACTCTCC257250.010.32848311
GGAGGTC267950.010.2007754
TGTATCC117050.010.0544033
TCCTAGG112100.09.9962597
TGGCATA194500.09.97972134
GTGGCGA149650.09.8001532
CGACCCG149150.09.7862646
GTATCCT117350.09.7798964
Produced by FastQC (version 0.11.5)