FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47912127
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG686450.14327270421536492No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA533450.11133924402896996No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG490250.1023227376233996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG46900.015.87251226
GGGAGGT324850.015.1650793
GGAGGTC349750.013.8834164
ATATAGC62500.013.6469356
GAGGTCA369800.013.1692035
GGTCACT361950.012.8521037
AGGTCAC373350.012.8512976
TGTCGTT59150.012.81689228
GTCGTTC60900.012.67050529
GCTAAGT61650.012.66699814
GTCACTC372150.012.5914548
TGGGAGG302250.012.1601522
ATAGGGG286700.012.08888438
AGCTTAG288800.012.05136845
GGCATAG288050.011.9300235
ACTCTCC393900.011.7819511
TCGTTCC66900.011.50107430
CGTTCCC68300.011.33204731
CACTCTC412150.011.26617810
GAGCTTA308250.011.16741944
Produced by FastQC (version 0.11.5)