FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48325246
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2127650.4402771172649592No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1028700.21287010106477264No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA841270.17408499068995945No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG798070.16514556387359103No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT575790.11914890200455472No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG528020.10926379971247327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT495550.022.0313871
CCGGCTT562050.021.8984762
CGGCTTC612050.020.2785823
TTCACGC597650.019.9956577
TCACGCT606800.019.6051778
AGACCGC267600.019.4332074
CAGACCG279250.018.8157883
GACCGCG281850.018.4187685
ACCGCGT283350.018.416526
ACGCTCA682300.017.59070410
CACGCTC690000.017.440049
GGCTTCA743850.017.0816174
TTCGACG13800.016.7880766
TCAGACC319700.016.7798672
CCGCGTT311750.016.7315857
CGCGTTC316600.016.4894818
CGTTACG11400.016.40213821
GCTTCAC763850.016.3546245
TCGCGTG7300.015.7141212
AACGCTA753750.015.64092223
Produced by FastQC (version 0.11.5)