FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47344990
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG980970.20719615739701286No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT840200.17746333878199153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT234600.021.8523431
CCGGCTT272250.021.4625322
TCACGCT278550.020.7426668
TTCACGC279600.020.6245377
ACGCTCA296500.019.79803810
AACGCTA283600.019.66888623
ACGCTAC286400.019.45304124
AGACCGC226600.018.9052164
CGCTACC297250.018.82605225
CGGCTTC321050.018.816923
ACCGCGT228050.018.6862726
GACCGCG231200.018.4220455
AAAACGC306450.018.35641921
CAGACCG234150.018.3149433
GAAAACG309200.018.03277420
CCGCGTT238850.017.86967
CGCGTTC239350.017.851078
CACGCTC329950.017.7159239
AAACGCT321150.017.52327222
GAACGAT246800.017.40335845
Produced by FastQC (version 0.11.5)