FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54610052
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174950.012.88619245
GGCATAG179100.012.5287135
ATAGGGG192450.011.78825338
GGGAGGT321650.011.5404273
GAGCTTA199850.011.29063244
TGGCATA200750.011.25602234
ACCCGGC96450.011.0793135
GTCACTC308750.010.8640598
GGAGGTC326150.010.7673044
CATAGGG210150.010.68828337
ACTCTCC315550.010.66558811
GGTCACT320450.010.3971977
GCATAGG219650.010.23625536
ATAGCTC100100.010.23019939
CGTAGGG107900.09.9078437
GCGTAGG110800.09.68914436
GAGGTCA361650.09.65438655
GGCGTAG110900.09.63981835
AGCATAG114600.09.54103945
TCACCCG117450.09.4814253
Produced by FastQC (version 0.11.5)