FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43551225
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1198520.27519776998235984No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTGG747710.17168518221932907No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT727580.1670630389845521No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT670150.15387626869278648No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA568530.13054282629248662No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG522950.12007698979764633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT275000.021.85991
TTCGACG20550.021.5669296
CCGGCTT318550.021.3746512
TTCACGC331200.020.0718147
TCACGCT341800.019.4032698
CGAACGA50100.018.77427732
CGGTATA30250.018.22068220
AGACCGC191700.018.1583294
ACGAACG52600.018.05293831
CAGACCG196100.017.923023
CGGCTTC401900.017.7199883
CGTTACG15350.017.29507821
TCGACGA27100.016.7687827
ACGCTCA419450.016.34760710
AACGCTA402450.016.19572423
GCGGACT232900.016.1537912
ACGCTAC405600.016.07578724
GGCTTCA458000.015.8147824
GTTCGAC26400.015.7679615
GTTAGTT198000.015.76218844
Produced by FastQC (version 0.11.5)