FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44385559
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1784310.40200237198769984No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG593180.13364256604270772No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA519070.11694569398123386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT314350.027.97791145
AGAACGA399850.023.11513344
AGACCGC407600.022.7102074
CAGACCG412250.022.4868183
GACCGCG425750.021.7261775
ACCGCGT434850.021.3219176
TTCGACG21900.021.2661726
CCGCGTT441600.020.990917
CGCGTTC443600.020.9013428
AAGAACG446850.020.64862343
TCAGACC455450.020.6108112
AATACGG462650.019.97749132
TACGGAG462300.019.88068234
ATACGGA464050.019.86388833
GCGTTCT468100.019.8410289
CTCAGAC507150.019.4820691
CAATACG480350.019.29288331
ACGGAGA492650.018.63765135
CCCAATA501150.018.59168229
CGGAGAG504400.018.13211836
Produced by FastQC (version 0.11.5)